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Danaher Inc fast protein liquid chromatography system
FIG. 4. Size exclusion chromatographic analysis of purified recombinant maize E2. Superose 6 <t>chromatography</t> was used to resolve high molecular mass standards (mean of three separate sam- ples) for the standard curve: blue dextran, 2000 kDa; thyroglobulin, 669 kDa; ferritin, 440 kDa; catalase, 232 kDa; aldolase, 158 kDa. The arrow indicates the position at which recombinant E2 eluted from at least three different preparations, and the inset shows a typical A280 elution profile.
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Danaher Inc size exclusion fast protein liquid chromatography
FIG. 4. Size exclusion chromatographic analysis of purified recombinant maize E2. Superose 6 <t>chromatography</t> was used to resolve high molecular mass standards (mean of three separate sam- ples) for the standard curve: blue dextran, 2000 kDa; thyroglobulin, 669 kDa; ferritin, 440 kDa; catalase, 232 kDa; aldolase, 158 kDa. The arrow indicates the position at which recombinant E2 eluted from at least three different preparations, and the inset shows a typical A280 elution profile.
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FIG. 4. Size exclusion chromatographic analysis of purified recombinant maize E2. Superose 6 chromatography was used to resolve high molecular mass standards (mean of three separate sam- ples) for the standard curve: blue dextran, 2000 kDa; thyroglobulin, 669 kDa; ferritin, 440 kDa; catalase, 232 kDa; aldolase, 158 kDa. The arrow indicates the position at which recombinant E2 eluted from at least three different preparations, and the inset shows a typical A280 elution profile.

Journal: The Journal of biological chemistry

Article Title: The dihydrolipoamide S-acetyltransferase subunit of the mitochondrial pyruvate dehydrogenase complex from maize contains a single lipoyl domain.

doi: 10.1074/jbc.274.31.21769

Figure Lengend Snippet: FIG. 4. Size exclusion chromatographic analysis of purified recombinant maize E2. Superose 6 chromatography was used to resolve high molecular mass standards (mean of three separate sam- ples) for the standard curve: blue dextran, 2000 kDa; thyroglobulin, 669 kDa; ferritin, 440 kDa; catalase, 232 kDa; aldolase, 158 kDa. The arrow indicates the position at which recombinant E2 eluted from at least three different preparations, and the inset shows a typical A280 elution profile.

Article Snippet: Size Exclusion Chromatography—The native size of purified recombinant maize E2 was determined using a Superose 6 column in conjunction with a fast protein liquid chromatography system (FPLC; Amersham Pharmacia Biotech).

Techniques: Purification, Recombinant, Chromatography